Cell Biology

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• Prof. Marie Jose Tassignon of Belgium visited Raghudep Eye Clinic • Team Raghudeep bagged the 2nd Prize in the "Innovative Video" category at the Film Festival, Annual Meeting of European Cataract and Refractive Surgeons (ESCRS), Sept 2011 meeting in Vienna, Austria. • Annual Phacoemulsification Workshop - Colloquium 2011, successfully conducted on 3 and 4th September in Ahmedabad. More than 300 delegates from different parts of the country attended the Workshop. • Donations made to Iladevi Cataract and IOL Research Center are eligible for exemption by 200% under section 35(i)(ii) of the Income Tax Act.• Dr. Abhay R Vasavada recieves the Binkhorst Medal at the ASCRS meeting in San Diego, March 25-29, 2011• Novel Glaucoma Therapy – Selective Laser Trabeculoplasty, Now Available At Raghudeep Eye Clinic; The First Time In Gujarat• Dr. Abhay Vasavada was recently awarded the 2010 APACRS LIM Lecture Award at Cairns, Australia.• Dr. Vaishali Vasavada was awarded with the prestigious Sante Vision Award for "Best Paper Cataract Session" by Mr. Mukhopadhaya, Chief Justice Gujarat High Court at 69th All India Ophthalmological Conference held at Ahmedabad from 3-6th Feb 2011• Team Raghudeep makes several scientific paper and poster presentations at the 69th AIOC conference held between 3rd and 6th Feb 2011 at Ahmedabad• Prof. Marie Jose Tassignon of Belgium visited Raghudep Eye Clinic and performed live surgery to demonstrate the "Bag-in-the-lens" IOL, Jan 2011• Indian Institute of Management-Ahmedabad(IIM-A) selects Raghudeep Eye Clinic as their Management Case Study(2009).• Raghudeep eye clinic is an ISO 9001:2008 certified hospital.• Team Raghudeep bags awards for several paper, poster and video film presentations at the ASCRS Annual Meeting held in March 2011 in San Diego, USA• Raghudeep Eye Clinic services now at Deesa, North Gujarat.

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Cell Biology

The department works with cell line of Lens epithelial cell (LEC) and is equipped with modern equipments such as various types of microscopes, tissue culture and tissue processing facilities.

Using the cultured LECs, in an experimental study various agents were screened for their inhibitory effect on LECs as possible therapies for PCO. We have a provisional patent applied for the possible prevention of PCO using inhibitors of cell proliferation.

We are focusing towards the role of epithelial mesenchymal transition (EMT) and disruption of cell polarity in the formation of plaque and PCO. We are also studying the utilization of natural compounds for prevention of PCO.

Research is also directed towards understanding the lens protein modifications at the molecular level during aging and cataractogenesis. The cytoskeletal proteins in (vimentin, actin, laminin) the cataractous epithelia are studied using immunohistochemistry

Completed Projects :

Sr No.	Project Title	Duration	Year	Agency
1	A possible role of intracellular calcium in cataractogenesis: comparison of calcium levels in different types of cataract.	4 year	2001–2004	CSIR, NewDelhi.
2	Novel molecular diagnostics for eye diseases and low vision enhancement devices.	3 year	2002–2005	CSIR, NewDelhi.
3	Pharmacological and molecular biological approaches to prevent Posterior Capsular Opacification (PCO).	3 year	2002–2005	DST, New Delhi
4	Trial of square and round edge IOL in ECCE	2 year	2002–2004	Mr.David Spalton, St. Thomas Hospital, London,UK.
5	Cytological, biochemical and pharmacological approach to dissolve Corticocapsular adhesions (CCA)	3 year	2006-2009	DST/SERC Division, New Delhi
6	Evaluation of total antioxidant status and antioxidative enzymes in congenital and senile cataracts	3 year	2005-2007	ICMR, New Delhi
7	Cell polarity and lens epithelial cell plasticity: Implication in the aetiology of cataract and post-operative capsular opacification (PCO).	3 years	2008- 2011	CSIR, New Delhi

Publications :

1. Johar SR, Vasavada AR, Praveen MR, Pandita D, Nihalani B, Patel U, Vemuganti G.

Histomorphological and immunofluorescence evaluation of bimanual and coaxial phacoemulsification incisions in rabbits.
J Cataract Refract Surg. 2008 Apr;34(4):670-6.

2. Rajkumar S, Praveen MR, Gajjar D, Vasavada AR, Alapure B, Patel D, Kapur S.

Activity of superoxide dismutase isoenzymes in lens epithelial cells derived from different types of age-related cataract.
J Cataract Refract Surg. 2008 Mar;34(3):470-4.

3.Gajjar D, Praveen MR, Vasavada AR, Pandita D, Vasavada VA, Patel DB, Johar K, Raj S.

Ingress of bacterial inoculum into the anterior chamber after bimanual and microcoaxial phacoemulsification in rabbits. J Cataract Refract Surg. 2007 Dec;33(12):2129-34.

4.Johar K, Vasavada AR, Tatsumi K, Dholakia S, Nihalani B, Rao SS.

Anterior capsular plaque in congenital cataract: occurrence, morphology, immunofluorescence, and ultrastructure.
Invest Ophthalmol Vis Sci. 2007 Sep;48(9):4209-14.

5.Vasavada AR, Praveen MR, Pandita D, Gajjar DU, Vasavada VA, Vasavada VA, Raj SM, Johar K.

Effect of stromal hydration of clear corneal incisions: quantifying ingress of trypan blue into the anterior chamber after phacoemulsification.
J Cataract Refract Surg. 2007 Apr;33(4):623-7.

6.Gupta PD, Johar K Sr, Nagpal K, Vasavada AR.

Sex hormone receptors in the human eye.
Surv Ophthalmol. 2005 May-Jun;50(3):274-84. Review.
Vasavada AR, Raj SM. Cataract treatment where resources are scarce.
Lancet. 2005 Feb 12-18;365(9459):550-1.

7.Johar SR, Rawal UM, Jain NK, Vasavada AR.

Sequential effects of ultraviolet radiation on the histomorphology, cell density and antioxidative status of the lens epithelium--an in vivo study.
Photochem Photobiol. 2003 Sep;78(3):306-11.

8.Vasavada AR, Thampi P, Yadav S, Rawal UM.

Acid phosphatase and lipid peroxidation in human cataractous lens epithelium. Indian J Ophthalmol. 1993 Dec;41(4):173-5.

9.Vasavada AR, Cherian M, Yadav S, Rawal UM.

Lens epithelial cell density and histomorphological study in cataractous lenses. J Cataract Refract Surg. 1991 Nov;17(6):798-804.

10.Johar SR, Savalia NK, Vasavada AR, Gupta PD.

Epidemiology based etiological study of pediatric cataract in western India.
Indian J Med Sci. 2004 Mar;58(3):115-21.

11. Kalariya N, Rawal UM, Vasavada AR.

Human lens epithelial layer in cortical cataract.
Indian J Ophthalmol. 1998 Sep;46(3):159-62.

12. Gajjar D, Patel D, Alapure B, Praveen MR, Patel A, Johar K Sr, Vasavada AR.

Rapid action of oestradiol against hydrogen peroxide-induced oxidative stress in cataractous lens epithelium: an in vitro study. Eye (Lond). 2009 Jun;23(6):1456-63.